Glycerol-Glo™ Assay
Simple, Sensitive Glycerol Detection Assay for a Variety of Biological Samples
- Works with lysed cells, tissues, medium and serum samples
- Perform directly in-well
- Linear glycerol detection up to 80µM
Catalog Number:
Size
Catalog Number: J3150
Catalog Number: J3151
Rapid and Sensitive Glycerol Detection in Biological Samples
The Glycerol-Glo™ Assay is a bioluminescent assay for rapid and sensitive measurement of glycerol in a variety of biological samples, including cells grown in monolayer or 3D structures, cell culture medium, tissues and serum samples. Glycerol is often measured as the product of lipolysis, where it is released from triglycerides. Glycerol is also a substrate or product of many other enzymatic or metabolic processes that can be studied with the glycerol assay. Any processes that result in changes in glycerol concentration, both extracellular and intracellular, can be studied with the Glycerol-Glo™ Assay.
How the Assay Works
The Glycerol-Glo™ Assay measures glycerol in a coupled reaction scheme that links the production of NADH to the activation of a proluciferin that produces light with luciferase. Glycerol kinase and glycerol-3-phosphate dehydrogenase are used to generate NADH.
In the presence of NADH, Reductase enzymatically reduces a proluciferin Reductase Substrate to luciferin. Luciferin is detected in a luciferase reaction using Ultra-Glo™ Luciferase and ATP and the amount of light produced is proportional to the amount of glycerol in the sample.
Simple, Flexible Sample Preparation
Glycerol Release from Adipocytes
The Glycerol-Glo™ Assay can be used with cultured cells, including 3D cell culture models, homogenized tissue samples, cell culture medium samples and serum or plasma samples.
No organic extraction is required. The Glycerol Lysis Reagent effectively extracts glycerol from your sample in a single reagent addition without extreme heat, centrifugation steps or multi-step organic extraction protocols.
Protocols
Complete Protocol
Protocolos Rápidos
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
Reductase Substrate |
G885A | 1 × 55μl | |
Glycerol Lysis Solution |
J315A | 1 × 10ml | |
Kinetic Enhancer |
J316A | 1 × 50μl | |
Glycerol Detection Solution |
J317A | 1 × 5ml | |
Glycerol Standard |
J324A | 1 × 500μl | 20mM |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
Reductase Substrate |
G885B | 2 × 275μl | |
Glycerol Lysis Solution |
J315B | 1 × 100ml | |
Kinetic Enhancer |
J316B | 1 × 500μl | |
Glycerol Detection Solution |
J317B | 1 × 50ml | |
Glycerol Standard |
J324A | 1 × 500μl | 20mM |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.
Resources
Related Products
Productos Similares
Triglyceride-Glo™ Assay
Detecta los niveles de triglicéridos midiendo el glicerol que se libera de una reacción enzimática con una lipasa.
J3160, J3161
Cholesterol/Cholesterol Ester-Glo™ Assay
Mide el colesterol por asociación con la producción de NADH y la activación de proluciferina que produce luz con una enzima luciferasa.
J3190, J3191
Lumit® Glucagon Immunoassay
Mide el glucagón de forma cuantitativa a partir de muestras de cultivos celulares o de secreción de islotes mediante un protocolo rápido y sencillo que no requiere lavado.
W8020, W8022
Lumit® Insulin Immunoassay
Quantitatively measures insulin from cell culture or islet secretion samples using a fast, easy no-wash protocol.
W8010, W8012
Usado con frecuencia junto con
GloMax® Discover System
Lector de microplacas de alto rendimiento para la detección de luminiscencia, fluorescencia y absorbancia.
GM3000
RealTime-Glo™ MT Cell Viability Assay
Método bioluminiscente que permite monitorizar la cinética de viabilidad de cultivos celulares durante 72 horas.
G9711, G9712, G9713
LDH-Glo™ Cytotoxicity Assay
Ensayo LDH basado en bioluminiscencia para detectar de forma sensible la citotoxicidad en muestras con bajo número de células, incluidos los microtejidos 3D.
J2380, J2381
CellTiter-Glo® 2.0 Cell Viability Assay
Versión actualizada del CellTiter-Glo® Cell Viability Assay con una estabilidad del reactivo mejorada. Cuantifica la proliferación celular basándose en la detección de ATP.
G9241, G9242, G9243
