Monster Green® Fluorescent Protein phMGFP Vector
Improved Synthetic GFP Reporter with Greater Fluorescent Intensity and Reduced Cytotoxicity
- Visualize low-level expression in situ using fluorescence microscopy, imagers or FACS®
- Create fusion proteins for imaging and localization studies using standard FITC detection
- Transfect at high efficiencies for precloning confirmation studies
Catalog Number:
Size
Catalog Number: E6421
The phMGFP Vector contains the open reading frame for the Monster Green® Fluorescent Protein cloned into a mammalian expression vector. The Monster Green® Fluorescent Protein is encoded by an improved synthetic version of the green fluorescent protein gene originally cloned from Montastrea cavernosa (Great Star Coral). The synthetic gene (hMGFP) expresses a 26kDa protein that shows improved fluorescence intensity compared to the native gene. Furthermore, the hMGFP gene has been codon optimized and cleared of most consensus sequence transcription factor binding sites to ensure reliability and high levels of expression.
The Monster Green® Fluorescent Protein encoded by the hMGFP gene is an ideal fluorescent reporter, providing high-level fluorescence and reducing cytotoxicity. Monster Green® Fluorescent Protein generally fluoresces at least 20% brighter than other commercially available green fluorescent proteins (GFPs) and also minimizes cellular perturbations, offering flexibility when working with transient and stable expression assays.
Monster Green® Fluorescent Protein phMGFP Vector GenBank® Accession Number AY218848 and vector sequence text file.
Protocols
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
Monster Green® Fluorescent Protein phMGFP Vector |
E642A | 1 × 20μg |
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
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