Promoter-Driven Control Firefly Luciferase Vectors
Vectors That Are Co-transfected into Cells as Controls in Dual-Reporter Assays
- Co-transfect into cells where Renilla or NanoLuc® luciferase is the experimental reporter gene
- SV40, PGK and TK promoter options offer different expression levels suitable for most dual-reporter assays
Catalog Number:
Choose a promoter
Size
Catalog Number: E6681
Catalog Number: E5011
Catalog Number: E5061
The promoter-driven firefly control vectors are commonly co-transfected with experimental NanoLuc® or Renilla luciferase vectors for use in dual-reporter assays such as the Dual-Luciferase® or Dual-Glo® Reporter Assay Systems. The control vectors should give an almost invariant level of activity, while the experimental Renilla vector varies with treatment. The luc2 gene that encodes firefly luciferase is optimized for mammalian expression. If the CMV promoter is desired, the pGL4.50[luc2/CMV/Hygro] and pGL4.51[luc2/CMV/Neo] Vectors are useful for tagging a cell line and offer a selectable marker for creating stable transfectants.
Applications
- Transcription regulation.
- Virus-cell interactions.
- Compound screening.
- Post-translational modifications.
- GPCR signaling.
- Cell signaling.
- Promoter analysis.
About the pGL4 Reporter Vectors
The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Firefly luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.
Sequence Information
pGL4.13[luc2/SV40] Vector GenBank® Accession Number AY738225 and vector sequence text file.
pGL4.53[luc2/PGK] Vector GenBank® Accession Number KM359768 and vector sequence text file.
pGL4.54[luc2/TK] Vector GenBank® Accession Number KM359769 and vector sequence text file.
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
pGL4.13[luc2/SV40] Vector |
E668A | 1 × 20μg |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
| pGL4.53[luc2/PGK] Vector | E501A | 1 × 20μg | 1μg/μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Contenido
| Item | Part # | Presentación |
|---|---|---|
| pGL4.54[luc2/TK] Vector | E506A | 1 × 20μg |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Resources
No related resources available
Related Products
Productos Similares
pGL4 in vivo Imaging Vectors
Vectores pGL4.50 y pGL4.51 con un nivel alto de expresión de luciferasa firefly en líneas celulares estables para su uso en estudios con animales.
E1310, E1320
Promoterless Firefly Luciferase Basic Vectors
Tres vectores reporteros pGL4 que expresan proteínas luciferasa con diferente estabilidad para investigar las regiones de control de la expresión génica.
E6651, E6661, E6671
Promoter-Driven Control NanoLuc® Luciferase Vectors
Tres vectores reporteros NanoLuc® con promotor CMV, PGK o TK. Co-transfección con vectores de luciferasa firefly para la normalización en ensayos de doble reportero.
N1441, N1501, N1091
Usado con frecuencia junto con
Dual-Luciferase® Reporter Assay System
Detección consecutiva de las actividades luciferasa firefly y Renilla en una sola muestra. Requiere luminómetro con 2 inyectores.
E1910, E1960, E1980
Dual-Glo® Luciferase Assay System
Detección de forma consecutiva de las actividades luciferasa firefly y Renilla en una sola muestra. Vida media de la señal de dos horas. No requiere inyectores.
E2920, E2940, E2980
Nano-Glo® Dual-Luciferase Reporter Assay System
Detección ultrasensible de la actividad luciferasa firefly y NanoLuc® en una sola muestra.
N1610, N1620, N1630, N1650, N1521, N1531, N1541, N1551
Bright-Glo™ Luciferase Assay System
Ensayo reportero sensible para un procesamiento continuo en sistemas robotizados.
E2610, E2620, E2650
Steady-Glo® Luciferase Assay System
Ensayo reportero sensible con luminiscencia de larga duración para el procesamiento por lotes en placas multipocillo.
E2510, E2520, E2550
ONE-Glo™ Luciferase Assay System
Ensayo robusto de luciferasa firefly con un rendimiento mejorado para aplicaciones de alto y máximo rendimiento.
E6110, E6120, E6130
GloMax® Discover System
Lector de microplacas de alto rendimiento para la detección de luminiscencia, fluorescencia y absorbancia.
GM3000
