FuGENE® SI Transfection Reagent
Versatile Transfection Reagent for siRNA, miRNA and Other Small RNA Molecules
- High-efficiency transfection in both challenging and routine eukaryotic cell lines
- Maximum knockdown with low toxicity—fewer cells required
- Simple protocol with minimal optimization
Catalog Number:
Size
High-Efficiency, Gentle Reagent for Small RNA Delivery
FuGENE® SI is an innovative, 100% synthetic, multicomponent transfection reagent designed for the delivery of siRNA, miRNA and other small RNA molecules. FuGENE® SI provides high-efficiency delivery of RNA into a wide range of eukaryotic cell lines while providing the gentle, low-toxicity transfection you expect from FuGENE® reagents. Obtain maximum knockdown efficiencies while minimizing impact on cell health, allowing you to get the most out of transfection-based experiments.
Animal-free reagents and strong performance make FuGENE® SI ideal for a variety of RNA transfection applications from functional genomics to siRNA library screens.
The quick and easy protocol allows you to obtain superior results with minimal optimization, freeing-up valuable lab time.
Gentle and Nontoxic siRNA Transfection
FuGENE® SI Transfection Reagent delivers gentle and nontoxic siRNA transfection. HEK293-GFP and NIH3T3-GFP cell lines (Cell Biolabs) were seeded in 96-well plates according to user guides and subsequently transfected with 0.2, 1.0 or 5pmol of GFP targeting siRNA or negative control (Dharmacon™, Horizon) along with 0.3µl of FuGENE® SI Transfection Reagent. Cells were then analyzed via flow cytometry 48 hours post-transfection to measure total cell viability. Graphs show the gentle and nontoxic transfection of siRNAs using FuGENE® SI.
Superior Knockdown Efficiency
FuGENE® SI outperforms Lipofectamine™ RNAiMAX in siRNA knockdown efficiency. HEK293-GFP and NIH3T3-GFP cell lines (Cell Biolabs) were seeded in 96-well plates according to user guides and subsequently transfected with 0.2, 1.0 or 5pmol of GFP targeting siRNA or negative control (Dharmacon™, Horizon) along with 0.3µl of FuGENE® SI Transfection Reagent or 0.3µl of Lipofectamine™ RNAiMAX (Life Technologies). Cells were then analyzed via flow cytometry 48 hours post-transfection to measure % knockdown of GFP and total cell viability. Graphs show the superior knockdown performance of FuGENE® SI versus Lipofectamine™ RNAiMAX in HEK293 and NIH3T3 cells.
FuGENE® SI outperforms Lipofectamine™ RNAiMAX in siRNA knockdown efficiency, as confirmed by Western blot. Human MDA-MB-468 cells were reverse-transfected in 6-well plates with 40pmol of the indicated targeting siRNAs or negative/positive control (Silencer® Select siRNA, Invitrogen) utilizing either 7.5µl of FuGENE® SI Transfection Reagent or 7.5µl of Lipofectamine™ RNAiMAX per well. The cells were collected 72 hours after transfection and analyzed via Western blot for expression of target genes STAT3 and CEBPB. The Western blot shown here demonstrates the robust, superior knockdown with FuGENE® SI as compared to the market leading competitor.
Efficient Transfection of Labeled Small RNAs
FuGENE® SI provides efficient transfection of labeled small RNAs. A549, COS-7, HEK293 and HeLa cells plated in 96-well microplates were transfected with 0.4µl of FuGENE® SI or a no-reagent negative control and various amounts of FITC-labeled negative control siRNA. Twenty-four hours after transfection, the cells were analyzed via flow cytometry for FITC-positive signal.
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
FuGENE® SI Transfection Reagent |
E931A | 1 × 1ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
FuGENE® SI Transfection Reagent |
E931A | 5 × 1ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
No related resources available
Related Products
Productos Similares
FuGENE® 4K Transfection Reagent
Reactivo de transfección multicomponente más nuevo y avanzado, 100% sintético.
E5911, E5912
FuGENE® HD Transfection Reagent
Rendimiento mejorado en una amplia gama de tipos celulares, incluidas líneas celulares difíciles de transfectar.
E2311, E2312
FuGENE® 6 Transfection Reagent
Rendimiento probado y robusto. La mejor opción para un uso diario con líneas celulares habituales.
E2691, E2692, E2693
ViaScript® mRNA Transfection Reagent
Evenly adjust expression across cell population by proportionately changing amount of reporter mRNA transfected.
EV4961, EV4965, NE1100
ViaFect™ Transfection Reagent
Transfección de alta eficacia y baja toxicidad de muchos tipos celulares, incluyendo las células iPS.
E4981, E4982
ProFection® Mammalian Transfection System
Método basado en el uso de fosfato cálcico para la transfección estable o transitoria de células eucariotas.
E1200
Usado con frecuencia junto con
QuantiFluor® RNA System
Cuantificación fluorescente de pequeñas cantidades de ARN en solución.
E3310
RiboMAX™ Express RNAi System
Sintetiza dúplex de ARNsi para ARNi de mamíferos o ARNdc largo para ARNi de no mamíferos.
P1700
psiCHECK™-2 Vector
Vector reportero de luciferasa para monitorizar los efectos del ARN de interferencia.
C8021
pmirGLO Dual-Luciferase miRNA Target Expression Vector
Permite evaluar la actividad de los microARN (miARN) insertando sitios diana adyacentes al gen de la luciferasa firefly. Normaliza con Renilla luciferasa.
E1330
GoTaq® qPCR y RT-qPCR Systems
Master mix listas para su uso en qPCR o RT-qPCR basadas en dyes.
A6001, A6002, A6020, A6010
GloMax® Discover System
Lector de microplacas de alto rendimiento para la detección de luminiscencia, fluorescencia y absorbancia.
GM3000
GoTaq® Probe qPCR and RT-qPCR Systems
Master mix listas para su uso en qPCR o RT-qPCR basadas en sondas.
A6101, A6102, A6120, A6121, A6110
CellTiter-Glo® Luminescent Cell Viability Assay
Determine el número de células viables en el cultivo a partir de la cuantificación del ATP presente.
G7570, G7571, G7572, G7573
Steady-Glo® Luciferase Assay System
Ensayo reportero sensible con luminiscencia de larga duración para el procesamiento por lotes en placas multipocillo.
E2510, E2520, E2550
