MagneSil® Genomic, Fixed-Tissue System
Magnetic Method to Purify DNA from FFPE Tissue
- One-hour purification after overnight proteinase K digestion
- No centrifugation of lysate required
- Amplifiable DNA from 10µM FFPE tissue slices
Catalog Number:
Size
Catalog Number: MD1490
Amplification-Ready DNA from FFPE Tissue in 1 Hour
The MagneSil® Genomic, Fixed Tissue System provides a fast, simple technique for extracting genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue. After an overnight Proteinase K digestion, genomic DNA can be manually purified from FFPE thin tissue sections in less than an hour, and up to 12 samples can be processed at a time using the MagneSphere® Technology Magnetic Separation Stand (twelve-position) (Cat.# Z5342).
The optimized protocol and wash buffers yield genomic DNA that is largely free of small DNAs, which are potent inhibitors of PCR amplification. Amplifiable genomic DNA can be isolated from 10μm thin sections without centrifugation of the lysate prior to purification. Targets as large as 450–1,800bp can be amplified, and multiplex PCR can be performed using DNA purified with this system.
Protocols
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
Resin |
A825C | 1 × 900μl | View Product | |
|
Lysis Buffer |
A826C | 1 × 40ml | View Product | |
|
2X Wash Buffer |
A827C | 1 × 30ml | View Product | |
|
Elution Buffer |
A828C | 1 × 15ml | View Product | |
|
Incubation Buffer |
D920C | 1 × 35ml | ||
|
DTT |
V125A | 1 × 1.125ml | 1M | |
|
Proteinase K |
V302A | 2 × 10mg | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
See Protocol for detailed storage recommendations.
Resources
Otros
Related Products
Productos Similares
ReliaPrep™ FFPE gDNA Miniprep System
Purificación rápida y manual de ADN a partir de tejidos FFPE, sin utilizar disolventes orgánicos.
A2351, A2352
ReliaPrep™ FFPE Total RNA Miniprep System
ARN total amplificable, purificado a partir de tejidos FFPE sin disolventes orgánicos ni digestión durante la noche.
Z1001, Z1002
Maxwell® RSC DNA FFPE Kit
Extrae ADN a partir de muestras de tejido FFPE, utilizando el Maxwell® RSC Instrument.
AS1450, ASB1450
Maxwell® RSC RNA FFPE Kit
Purifica ARN a partir de secciones de tejido FFPE, utilizando el Maxwell® RSC Instrument.
AS1440
Usado con frecuencia junto con
GoTaq® G2 Master Mixes
GoTaq® G2 DNA Polymerase de segunda generación y alto rendimiento en una master mix lista para su uso.
M7822, M7823, M7832, M7833
GoTaq® G2 Hot Start Polymerase
Enzima GoTaq® G2 Hot-Start de alto rendimiento que permite una preparación a temperatura ambiente.
M7401, M7405, M7406, M7408
QuantiFluor® ONE dsDNA System
Cuantificación "add-and-read" de ADN de doble cadena. Incluye un dye de unión al ADN, lambda ADN y un tampón.
E4871, E4870
QuantiFluor® ONE dsDNA Dye
Standalone fluorescent dye for quantifying double-stranded DNA.
E4891
