Maxwell® HT ccfDNA Kit

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Automated High-Throughput Extraction of Cell-Free DNA from Plasma, Serum, Urine and CSF

  • Flexible processing of 0.2 to 4ml plasma (or up to 8ml with additional buffer purchased separately)
  • Highest ccfDNA yields for demanding rare target detection
  • ccfDNA ready for qPCR, ddPCR and targeted and whole genome sequencing

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Catalog number selected: A6030

$ 1,640.00
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Maxwell® HT ccfDNA Kit
1 × 96 preps
$ 1,640.00
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Ready-to-Go ccfDNA Extraction Kit, Expert Support Available 

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The Maxwell® HT ccfDNA Kit is compatible with automation on all major platforms, including Hamilton Star/Nimbus, Tecan Freedom Evo/Fluent, Biomek i5/i7, Eppendorf epMotion, and particle movers such as KingFisher.

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When you purchase a Promega extraction solution, you gain free access to our elite Field Support Scientists, method development services, and technical support. Contact us if you need help setting up the Maxwell® HT ccfDNA Kit on your robot.

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With decades of experience in building personalized automation solutions, we can help you customize the scale, application, and chemistry, regardless of platform, exactly to your needs—the only limit is your imagination.

Automated Purification of High-Quality, Amplifiable DNA

The Maxwell® HT ccfDNA Kit can be used for high-throughput, automated purification of circulating cell-free DNA (ccfDNA) from up to 96 samples of plasma with input volumes of 1.0–8.0ml.

Download Protocol
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Quantitation of isolated DNA by qPCR. DNA isolated from various input volumes using the Maxwell® HT ccfDNA Kit and the Qiagen QIAamp® ccfDNA/RNA kit is quantitated using qPCR. Yields using the Promega system are consistently higher when similar input volumes are used. Only the Promega system is capable of starting from as much as 8.0ml of input volume.

High-Throughput Automated ccfDNA Extraction from Plasma, Serum, Urine and CSF

The posters and application notes listed below contain protocols and example data using the Maxwell® HT ccfDNA Kit for automated purification of circulating, cell-free DNA from various body fluids on different robotic systems.

DNA Extraction Protocols image-56882596

Circulating Cell-Free DNA Resources

ccfDNA Webinar Series

Register for one of our on-demand webinars to learn more about trends in ccfDNA research. From "What is ccfDNA?" to advanced applications, this series covers all the basics and more, with an emphasis on optimizing extraction and purification of circulating DNA.

Part 1: ccfDNA 101: Emerging Trends in Oncology Research

Part 2: ccfDNA Workflows: Honing in on the Target

Part 3: ccfDNA In the Lab: Optimizing Purification for Sequencing

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Specifications

What's in the box?

Item Part # Size Available Separately

Maxwell® Resin E

MC148B 1 × 20ml

Binding Buffer (BBC)

MC1362 1 × 450ml View Product

Wash 1 (CWF)

A3091 1 × 125ml View Product

Wash 2 (CWG)

A3101 1 × 150ml View Product

PCR Elution Buffer (EBE)

MC151B 1 × 24ml View Product

NGS Elution Buffer (EBF)

MC152B 1 × 24ml View Product

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Patents and Disclaimers

U.S. Pat. No. 6,855,499 and other patents.

Resources

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