MagneSil® Total RNA mini-Isolation System
Fast, Automated RNA Isolation from Small Amounts of Cells, Tissue or Blood
- Liquid handler-compatible
- Concentrated RNA eluted in as little as 15μl
Catalog Number:
Size
Catalog Number: Z3351
The MagneSil® Total RNA mini-Isolation System provides a high-throughput 96-well format for fast, simple preparation of intact total RNA from small amounts of cell culture (≤1 × 105 tissue culture cells), tissue (≤2mg tissue lysate in 100μl) or freshly isolated whole blood (≤20μl). The protocol enables high-throughput automated purification on a variety of liquid-handling workstations. Isolation of total RNA in a 384-well format from cell culture (≤1 × 103 cells) and freshly isolated whole blood (≤5μl) also may be performed. Total RNA purification is achieved without vacuum filtration, centrifugation or precipitation. The 96-well procedure takes about 30 minutes to complete using a liquid-handling workstation.
Total RNA purified using this system is suitable for a variety of molecular biology applications including endpoint RT-PCR amplification and real-time RT-PCR.
- Only 30 minutes are required to process one 96-well plate, or 50 minutes for one 384-well plate on a Beckman Coulter Biomek® FX liquid handler.
- Elution volumes as low as 15μl provide concentrated RNA without the need for time-consuming vacuum concentration.
- DNase I treatment is included to remove genomic DNA contamination.
Protocols
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Nuclease-Free Water |
P1195 | 1 × 150ml | View Product |
|
RNA Lysis Buffer (RLA) |
Z305D | 1 × 100ml | View Product |
|
DNase Stop Solution |
Z312D | 1 × 26.5ml | |
|
Yellow Core Buffer |
Z317D | 1 × 25ml | |
|
MnCl2, 0.09M |
Z318D | 1 × 5ml | |
|
MagneSil® RNA Paramagnetic Particles |
Z336D | 1 × 20ml | |
|
DNase I (lyophilized) |
Z358A | 4 × 1 vial | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
No related resources available
Related Products
Productos Similares
SV 96 Total RNA Isolation System
Purifica ARN total a partir de 96 muestras en menos de una hora. Formato basado en el vacío.
Z3500, Z3505
Maxwell® RSC Instrument
Instrumento para la purificación automatizada de ácidos nucleicos, con cuantificación integrada. Sólo para uso en investigación.
AS4500
Maxwell® RSC SimplyRNA Cells Kit
Purifica ARN a partir de células, utilizando un bajo volumen de elución y el Maxwell® RSC Instrument.
AS1390
Maxwell® RSC RNA FFPE Kit
Purifica ARN a partir de secciones de tejido FFPE, utilizando el Maxwell® RSC Instrument.
AS1440
Usado con frecuencia junto con
RNasin® Ribonuclease Inhibitor
El producto más fiable para la inhibición de amplio espectro de las RNasas eucariotas comunes.
N2111, N2115, N2511, N2515, N2611, N2615
GoTaq® Hot Start Master Mixes
Master mix de PCR lista para su uso en la preparación de reacciones a temperatura ambiente.
M5122, M5123, M5132, M5133
