ImProm-II™ Reverse Transcriptase
Full-Length cDNA Synthesis for the Analysis of Rare or Long Messages
Catalog Number:
Size
Catalog Number: A3801
Catalog Number: A3802
Catalog Number: A3803
cDNA Synthesis for the Analysis of Rare or Long Messages
ImProm-II™ Reverse Transcriptase enables robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The ImProm-II™ Reverse Transcriptase can be used to reverse transcribe total RNA, poly(A)+ mRNA or synthetic transcript RNA templates.
Advantages- Full-Length RT-PCR: Reverse transcribe RNA templates up to 8.9kb.
- Microarray-Compatible: Incorporate regular and Cy®3- and Cy®5-modified nucleotides.
- Scalable and Flexible: Reaction volumes of 1–20μl can be used in subsequent PCR, and the optimized buffer also allows for coupled RT-PCR.
- Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl and 50mM DTT. A 25mM MgCl2 Solution also is included.
- Versatile: Use with your thermostable polymerase of choice for two-step RT-PCR.
- Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
cDNA Synthesis for Analysis of Rare or Long Messages
Protocols
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 1 × 750μl | 25mM | View Product |
|
ImProm-II™ 5X Reaction Buffer |
M289B | 1 × 100μl | ||
|
ImProm-II™ Reverse Transcriptase |
M314B | 1 × 10μl |
SDS
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Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351H | 1 × 1.2ml | 25mM | View Product |
|
ImProm-II™ 5X Reaction Buffer |
M289A | 1 × 600μl | ||
|
ImProm-II™ Reverse Transcriptase |
M314A | 1 × 100μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351H | 3 × 1.2ml | 25mM | View Product |
|
ImProm-II™ 5X Reaction Buffer |
M289C | 2 × 1,250μl | ||
|
ImProm-II™ Reverse Transcriptase |
M314C | 1 × 500μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
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