GoTaq® G2 DNA Polymerase
Next-Generation GoTaq® for Robust, Reliable PCR Amplification
- Full-length Taq DNA polymerase that exhibits 5´→3´ exonuclease activity
- Direct-to-gel convenience
- 5X Reaction Buffer with MgCl2 to simplify reaction setup
- Supplied at a concentration of 5u/μl
Catalog Number:
Size
Catalog Number: M7841
Catalog Number: M7845
Catalog Number: M7848
GoTaq® G2: Better Performance for PCR
GoTaq® G2 DNA Polymerase, the second generation of GoTaq® products, reliably amplifies a wide range of PCR templates and provides high-performing results due to improved manufacturing processes, increased reliability and consistency. GoTaq® G2 is supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification.
The enzyme is a full-length form of Taq DNA polymerase that exhibits 5´→3´ exonuclease activity. GoTaq® G2 DNA Polymerase is supplied with 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction Buffer. Both buffers contain MgCl2 at a concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction. The 5X Green GoTaq® Reaction Buffer has two dyes (blue and yellow) that separate during electrophoresis to indicate migration progress. The buffer also includes a compound that increases sample density so that samples can be loaded directly onto gels without the need for loading dye. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA from the PCR.
Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.
Consistent High Yields from Multiple Targets
GoTaq® G2 DNA Polymerase consistently produces high yields across multiple targets compared to other Taq polymerases. Three different targets were amplified with the GoTaq® G2 DNA Polymerase Green (G) and Colorless (C) buffers or competitor products (T, Q, L1, L2 and R) according to manufacturers’ recommended protocols using 3.3ng of input human DNA.
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
GoTaq® G2 DNA Polymerase |
M784A | 1 × 100u |
|
5X Green GoTaq® Reaction Buffer |
M791A | 1 × 1ml |
|
5X Colorless GoTaq® Reaction Buffer |
M792A | 1 × 1ml |
SDS
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Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
GoTaq® G2 DNA Polymerase |
M784B | 1 × 500u |
|
5X Green GoTaq® Reaction Buffer |
M791A | 4 × 1ml |
|
5X Colorless GoTaq® Reaction Buffer |
M792A | 4 × 1ml |
SDS
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Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
GoTaq® G2 DNA Polymerase |
M784B | 5 × 500u |
|
5X Green GoTaq® Reaction Buffer |
M791A | 20 × 1ml |
|
5X Colorless GoTaq® Reaction Buffer |
M792A | 20 × 1ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condiciones de Almacenaje
Resources
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