T7 RiboMAX™ Express Large Scale RNA Production System
Produces 5–8.5mg/ml RNA in 30 Minutes
- Consistent production of milligram amounts of RNA
- Minimal pipetting steps and setup time
- Efficiently transcribes DNA templates of varying sizes. Works with transcripts as short as 21bp.
- Animal-origin-free (AOF) and cGMP-manufactured version available for RNA therapeutic applications. Learn more.
Catalog Number:
Size
Catalog Number: P1320
Quickly Produce Milligram Amounts of RNA
The T7 RiboMAX™ Express Large Scale RNA Production System quickly generates large quantities of uncapped RNA transcripts suitable for in vitro or in vivo translation. RNA synthesized with the T7 RiboMAX™ Express Large Scale RNA Production System performs better for in vitro rabbit reticulocyte translation systems than RNA synthesized by standard methods. This is especially notable with high RNA concentrations that normally inhibit in vitro translation.
The T7 RiboMAX™ Express Large Scale RNA Production System offers a setup with minimal pipetting steps to increase efficiency and reduce potential errors. In order to provide this easy setup, a 2X transcription buffer that already includes all four rNTPs is included. The system also provides RQ1 RNase-Free DNase for the degradation of plasmid template after transcription.
Due to the combined 2X buffer and rNTPs, the T7 RiboMAX™ Express System is not recommended for the synthesis of RNA for applications that require capped RNA. For synthesis of capped RNA, please order the standard RiboMAX™ Large Scale RNA Production System—T7 (Cat.# P1300).
Applications of the T7 RiboMAX™ Express System include:
- Creation of molecular standards for RT-qPCR assays
- Generation of dsRNA and long RNAs for nonmammalian RNA interference studies
- In vitro synthesis of tRNA and rRNA, and RNA virus genomes and ribozymes
- Production of substrates for RNA splicing, studies of RNA secondary structure and RNA:protein interactions
- Synthesis of antisense RNA
RNA yield. The RNA yield from T7 RiboMAX™ Express in vitro transcription reactions incubated for the indicated time periods was determined by UV absorbance at 260nm. The numbers on top of each bar represent the RNA yield in micrograms resulting from in vitro transcription reactions with a volume of 20µl.
In the Literature
Development of mRNA manufacturing for vaccines and therapeutics: mRNA platform requirements and development of a scalable production process to support early phase clinical trials.
Duke Human Vaccine Institute at Duke University School of Medicine and Perelman School of Medicine at University of Pennsylvania together developed a scalable, GMP-compatible process for production of mRNA vaccines and therapeutics using Promega RiboMAX-related products.
Looking for a scalable IVT solution with animal-origin-free reagents? The RiboMAX™ AOF RNA Production System supports RNA synthesis from research to production.
Protocols
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
RQ1 RNase-Free DNase |
M610C | 1 × 110u | View Product |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
|
Enzyme Mix, T7 Express |
P132A | 1 × 100μl | |
|
Sodium Acetate, 3.0M (pH 5.2) |
P135A | 1 × 1ml | |
|
RiboMAX™ Express T7 2X Buffer |
P164A | 1 × 500μl | |
|
pGEM® Express Positive Control Template |
P256A | 2 × 5μg | View Product |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
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