FuGENE® HD Transfection Reagent Technical Manual
Instructions for Use of Product(s)
E2311, E2312
Literature # TM328
The FuGENE® HD Transfection Reagent is a nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Additionally, the FuGENE® HD Transfection Reagent has been shown to support transfection in chemically defined media and does not contain any animal-derived components.
Summary of Changes
The following changes were made to the 9/23 revision of this document:
1. Nontechnical edits to Sections 4, 5 and 7.
2. Updated font.
3. Updated formatting of storage conditions.
Revised 9/23.
Experienced User Protocols
