Solutions for Reliable & Accurate Protein Mass Spectrometry Sample Preparation
- How to optimize protein digestion and avoid unwanted side effects such as non-specific cleavages, non-enzymatic protein modification and extensive trypsin autoproteolysis
- How to complete the entire protein sample preparation within 1h
- How to increase protein sequence coverage using alternative proteases
- How to assess LC-MS/MS instrument performance
Summary
Common shortcomings of protein mass spec sample preparation include insufficient digestion, negative side effects including artificial modifications and non-specific cleavages, lengthy and laborious procedures, poor protein extraction and peptide recovery. This webinar will offer solutions to these shortcomings and discuss the advantages of our tools and reagents to achieve robust, reliable and accurate protein and proteome characterization with mass spectrometry. We will review the application of our tools and reagents to improve protein digestion and biotherapeutic protein characterization with peptide mapping.
Speaker
Chris Hosfield, PhD
Senior Research Scientist Promega Corporation
Chris Hosfield is a Senior Research Scientist in the Protein Analysis group at Promega. He has lead the development of tools for proteomics and antibody characterization, including PNGase F and IdeS and recombinant Asp-N proteases.
Chris has broad expertise working with proteolytic enzymes and preparing protein samples for analysis by mass spectrometry. Prior to joining Promega, Chris held senior scientist positions at several biotechnology companies where he applied proteomic methods to the discovery and development of both small molecules and biotherapeutics. He received his Ph.D. in biochemistry from Queen’s University.
Sergei Saveliev, PhD
Senior Research Scientist Promega Corporation
Sergei Saveliev is a Senior Research Scientist at Promega. He has a broad expertise in the life sciences. This includes knowledge of proteomics, enzymology, microbiology, and other fields. Lately, Sergei is primarily focused on development of tools for characterization of biotherapeutic proteins with mass spectrometry and RP-HPLC-UV.
Sergeis efforts are directed toward the development of solutions that help overcome the major challenges of protein mass spec sample analysis, including efficient proteolysis, standardization, and suppression of side effects of protein mass spec sample preparation. Sergei is the author of many publications in leading academic and industry journals.