Lumit® Immunoassay Labeling Kit and Detection Reagents
- Add-and-mix assay format—no washing steps, no immobilization to plates, beads or other surfaces, 30–90 minutes total time
- Simple luminescence-based detection
- Solution-based, sensitive assays with wide dynamic range
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Catalog Number: VB2500
Catalog Number: VB2010
Catalog Number: VB2020
Catalog Number: VB2030
Catalog Number: VB4050
Catalog Number: VB4060
Fast, Efficient Antibody and Protein Labeling
The Lumit® Immunoassay Labeling Kit allows you to label your antibodies and proteins with HaloTag®-SmBiT and HaloTag®-LgBiT to enable the development of a Lumit® Immunoassay. The Lumit® Detection Reagents A and B are specially designed to detect luminescence signal from Lumit® Immunoassays when using labeled primary antibodies.
Lumit® Immunoassays are based on NanoLuc® Binary Technology (NanoBiT® technology. NanoBiT, a structural complementation reporter designed for biomolecular interaction studies, is composed of two subunits, Large BiT (LgBiT) and Small BiT (SmBiT), that have been optimized for stability and minimal self-association due to weak affinity. When these subunits are fused to two interacting proteins, the subunits come into close proximity, reassemble into the functional enzyme and 'report' on the interaction.
How the Lumit® Immunoassay Labeling Kit and Detection Reagents Work
Lumit® Immunoassays are based on the chemical labeling of antibodies and proteins with SmBiT and LgBiT using HaloTag® technology. In the presence of an analyte, the two antibodies come into close proximity and allow SmBiT and LgBiT to form an active enzyme and generate a bright luminescence signal proportional to analyte concentration.
Lumit® Detection Reagents A and B are specially designed to detect luminescence signal from Lumit® Immunoassays when using labeled primary antibodies. Lumit® Detection Reagent A is used with biochemical and cell lysates, while Lumit® Detection Reagent B is specifically designed for analyte detection in cell culture media containing Fetal Bovine Serum (FBS) and for analyte detection directly in cell culture wells containing media and cells.
Chemical Labeling Using HaloTag® Technology
Labeling is a two-step process, where amine-reactive HaloTag® Succinimidyl Ester (O4) Ligand reacts with primary amines on lysine amino acids on the antibodies and proteins. Antibodies and proteins labeled with the HaloTag® ligand are then incubated with HaloTag®-LgBiT or HaloTag®-SmBiT fusion protein to make a covalent conjugate of antibody-HaloTag®-LgBiT or antibody-HaloTag®-SmBiT.
Simple Add-and-Mix Protocol, No Wash Steps Required!
The Lumit® Immunoassay does not require media removal or lysate transfer. The protocol simply involves addition of the sample to the labeled antibodies, addition of the detection reagent and reading of the luminescent signal—all in a single plate!
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
HaloTag® Ligand |
VB125A | 1 × 20μl | 20mM |
IGEPAL® CA-630 |
VB141A | 1 × 100μl | |
Lumit® HaloTag®-LgBiT |
VB211A | 1 × 350μl | 20μM |
Lumit® HaloTag®-SmBiT |
VB212A | 1 × 350μl | 20μM |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Lumit® Immunoassay Labeling System Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only and may link Lumit® HaloTag®-LgBiT and/or Lumit® HaloTag®-SmBiT fusion proteins to researcher-supplied moieties. No transfer or commercial use of this product or derivatives is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; and (2) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Notwithstanding the foregoing, researcher may use this product or derivatives in provision of services, information or data to third parties in exchange for consideration, provided that researcher does not transfer the product or derivatives. No other use of this product or its derivatives is authorized without the prior express written consent of Promega.
In addition, researcher must either:
(i) use Lumit®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(ii) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
U.S. Pat. Nos. 9,873,866 and 10,246,690; European Pat. Nos. 2341134, 2374875, 2395078, 2492342 and 2502990; Japanese Pat. Nos. 5680302 and 5840117 and other patents.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Lumit® Immunoassay Dilution Buffer A, 10X |
VB201A | 1 × 10ml |
Lumit® Detection Substrate A |
VB301A | 1 × 200μl |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Lumit® Immunoassay Dilution Buffer A, 10X |
VB201B | 1 × 100ml |
Lumit® Detection Substrate A |
VB301B | 2 × 1ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Lumit® Immunoassay Dilution Buffer A, 10X |
VB201B | 10 × 100ml |
Lumit® Detection Substrate A |
VB301C | 5 × 4ml |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Lumit® Detection Substrate B |
VB405A | 1 × 160μl |
Lumit® Detection Buffer B |
VB406A | 1 × 3.2ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Lumit® Detection Substrate B |
VB405B | 1 × 1.25ml |
Lumit® Detection Buffer B |
VB406B | 1 × 25ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
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