Wizard® MagneSil® Sequencing Reaction Clean-Up System
High-Throughput Purification of DNA from Sanger Sequencing Reactions
- Customized, walkaway methods available
- Purified products suitable for fluorescent sequencing
- Efficiently removes unincorporated ddNTPs from ProDye® Terminator Sequencing System
Catalog Number:
Size
Catalog Number: A1831
Catalog Number: A1832
Catalog Number: A1835
Ready-to-Go Kit, Expert Support AvailableÂ
The Wizard® MagneSil® Sequencing Reaction Clean-Up System is compatible with automation on all major platforms, including Hamilton Star/Nimbus, Tecan Freedom Evo/Fluent, Biomek i5/i7, Eppendorf epMotion, and particle movers such as KingFisher.
With decades of experience in building personalized automation solutions, we can help you customize the scale, application, and chemistry, regardless of platform, exactly to your needs—the only limit is your imagination.
Sequencing Reaction Clean-Up at Any Scale
The Wizard® MagneSil® Sequencing Reaction Clean-Up System is designed for high-throughput, automated purification of sequencing reactions, including ProDye® Terminator Sequencing System and BigDye® Terminator reactions. Cleanup is performed using MagneSil® GREEN Paramagnetic Particles with standard, nonskirted 96-well amplification plates. No user intervention is required from the time the plates are placed on the instrument until the samples are ready for loading onto the fluorescent DNA sequencer. Purified products are approved for fluorescent sequencing reactions (Phred* 20 quality scores ≥650 bases).
The system relies upon the MagnaBot® II for magnetic separation. The Plate Clamp 96 and Plate Stand are recommended for automated use because they ensure PCR plates are uniformly flat for liquid transfer on a robotic instrument.
*A Phred score is a widely recognized method to measure the quality of DNA sequences. Phred is a base-calling program for DNA sequence traces available from Codoncode Corporation.
Example Data
See an example protocol outlining this automation-friendly method in the Application Note: Wizard® MagneSil® Sequencing Reaction Clean-Up System for-up for Purification of ProDye® Terminator Sequencing Reactions
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
MagneSil® GREEN |
A8231 | 1 × 100ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
MagneSil® GREEN |
A8231 | 2 × 100ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
MagneSil® GREEN |
A8231 | 25 × 100ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
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Frequently Used With
MagnaBot® II Magnetic Separation Device
For high-throughput bioseparation using magnetic particles with a 96-well PCR plate.
V8351
Plate Clamp 96
Ensures that nonskirted PCR plates are uniformly flat for liquid transfer on a robotic platform.
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MagneSil® GREEN
High-throughput, automated purification of sequencing reactions.
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ProDye® Terminator Sequencing System
An improved method of fluorescent Sanger sequencing using a proprietary thermostable DNA polymerase included in the master mix.
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