Rabbit Reticulocyte Lysate, Nuclease-Treated
In Vitro Translation of mRNA and Characterization of mRNA Products
- Optimized for translation and ready to use
- Contains an energy-regenerating system, a mix of tRNAs, hemin, potassium chloride and magnesium acetate
Catalog Number:
Size
Catalog Number: L4960
Identify mRNAs, Characterize Protein ProductsÂ
Rabbit Reticulocyte Lysate System, Nuclease Treated can be used to identify mRNA species, characterize their protein products and investigate transcriptional and translational control. Rabbit Reticulocyte Lysate is prepared from New Zealand white rabbits using a standard protocol that ensures reliable and consistent reticulocyte production in each lot. After the reticulocytes are lysed, the extract is treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum. The lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors).
Learn More: See the Protein Expression chapter of the Protocols & Applications Guide for more information.
Choose Your Configuration: Learn more about custom options for this product at: www.promega.com/custom/Applications
- In vitro translation from mRNA, in vitro run-off transcripts or total RNA into protein
- Characterization of mRNA products
- Co-translational processing of secreted proteins by the addition of microsomal membranes to the translation reaction
- Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.
- Glass, C.A. and Pollard, K.M. (1990) Promega Notes 26, 6–9.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Reticulocyte Lysate, Nuclease Treated |
L416A | 5 × 200μl | ||
|
Amino Acid Mixture Minus Cysteine |
L447C | 1 × 50μl | 1mM | |
|
Luciferase Control RNA |
L456A | 1 × 10μl | View Product | |
|
Amino Acid Mixture Minus Leucine |
L995A | 1 × 50μl | 1mM | |
|
Amino Acid Mixture Minus Methionine |
L996A | 1 × 50μl | 1mM | View Product |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
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