HisLinkā¢ Protein Purification Resin
Resin to Purify His- or HQ-Tagged Protein from Cleared Lysate
- Purify polyhistidine- or HQ-tagged proteins from cleared lysate by standard column chromatography or from crude lysate in batch
- Suitable for low- to medium-pressure applications such as FPLC
- Large quantities of polyhistidine- or HQ-tagged protein can be purified per volume of resin used
Catalog Number:
Size
Catalog Number: V8821
Catalog Number: V8823
HisLink™ Protein Purification Resin is a macroporous silica resin modified to contain a high level of tetradentate-chelated nickel (>20mmol Ni/ml settled resin) for efficient capture and purification of bacterially expressed polyhistidine- or HQ-tagged proteins.
HisLink™ Resin may be used in either column or batch purification formats. Gravity feed of a cleared lysate over a HisLink™ column is sufficient for complete capture and efficient elution of polyhistidine- or HQ-tagged proteins; however, the resin also may be used with vacuum filtration devices (e.g., the Vac-Man® Vacuum Manifold, Cat.# A7231) to allow simultaneous processing of multiple columns.
HisLink™ Resin also is an excellent choice for affinity purification using low- to medium-pressure liquid chromatography systems such as fast performance liquid chromatography (FPLC). In batch format, HisLink™ Resin may be easily separated from the lysate without filtration, allowing processing of larger quantities of lysate and purification of protein from lysate that has not been cleared of insoluble cellular debris.
Note: Resin product volumes represent total volume of a 50% resin slurry.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
HisLink™ Protein Purification Resin |
V882A | 1 × 50ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. Nos. 7,112,552 and 7,354,750.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
HisLinkā¢ Protein Purification Resin |
V882C | 1 × 5ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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V8491
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G6280, G6270
Vac-ManĀ® Jr. Laboratory Vacuum Manifold
For rapid, effective nucleic acid purification. Process two samples at a time.
A7660, A3110
