OX40 Bioassay Technical Manual
Instructions for Use of Product(s)
JA2191, JA2195, JA3191, JA3195, JA2251, JA2255
Literature # TM581
The OX40 Bioassay is a bioluminescent cell-based assay that overcomes the limitations of existing assays. It can be used to measure the potency and stability of ligands or agonist antibodies that can bind and activate OX40. The assay consists of a genetically engineered Jurkat T cell line that expresses human OX40 and a luciferase reporter driven by a response element that can respond to OX40 ligand/agonist antibody stimulation. The OX40 Effector Cells are provided in thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell culture and propagation.
Summary of Changes
The following changes were made to the 6/25 revision of this document:
1. Removed text about the label in Section 3.
2. Removed an expired patent statement and updated two other patent statements.
3. Updated the fonts.
4. Made minor text edits.
Revised 6/25.