QuantiFluor® RNA Select System Technical Manual
Instructions for Use of Product(s)
E6640, E6780
Literature # TM773
The QuantiFluor® RNA Select System provides a sensitive, red‑fluorescent dye‑based method (648nmEx/665nmEm) for quantitating purified RNA over a broad linear range of 16–1,000ng per assay well (equivalent to 1.6–100ng/μl when using a 10μl sample). The dye exhibits high specificity for RNA with minimal background, is compatible with common laboratory buffers and the working solution is stable for >8 hours at 20–25°C when protected from light. Quantitation can be performed on single-tube fluorometers (e.g., Quantus™ Fluorometer) or can be scaled up for use on instruments that read multiwell plates (e.g., GloMax® Discover System, or any reader equipped with the appropriate excitation and emission wavelengths). Resulting data are suitable to prepare samples for downstream applications such as RT‑qPCR, RNA‑seq and library preparation.
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