ReliaPrep™ RNA Miniprep Systems
Efficiently Purify RNA from as Few as 100 Cells or 0.25mg Tissue
- Simple, 30-minute protocol
- Elutes in small volume, generating highly concentrated RNA
- No phenol/chloroform or β-mercaptoethanol required
Catalog Number:
Choose a sample type
Size
Catalog Number: Z6010
Catalog Number: Z6011
Catalog Number: Z6012
Catalog Number: Z6110
Catalog Number: Z6111
Catalog Number: Z6112
Recover Concentrated, Pure RNA from Precious Cell and Tissue Samples
Precious samples, sensitive assays, low copy messages – the effectiveness of RNA purification sets the stage for downstream results.
The ReliaPrep™ RNA Miniprep Systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes. The proprietary column/binding matrix efficiently captures RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15µl).
Using this membrane-based purification system, from 100 to 5 × 106 cultured cells or 0.25 to 20mg of tissue can be processed per purification. The system incorporates a DNase treatment step directly on the minicolumn membrane and effectively removes substances that can inhibit downstream assays. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, resulting in pure RNA that does not require additional purification or concentration of the RNA for use in demanding applications.
- Small sample size and low elution volume allows conservation of precious samples.
- Maximum sensitivity for downstream assays without worry of inhibition when measuring low-copy-number targets.
- Usable, concentrated RNA is eluted in as little as 7µl. No need to further concentrate samples.
- Rapid protocol and provided DNase reagents streamline laboratory processes.
- Purified RNA is suitable for many common downstream assays.
RNA from Cells
Higher RNA Yield
The data below show that the ReliaPrep™ Cell RNA Miniprep System efficiently isolates RNA from 100 to 1 x 106 HeLa cells with a higher RNA yield than the leading competitor. Next, the RNA yield from 100,000 cells of 4 different cell lines. More data on performance of the ReliaPrep™ Systems is available in this Application Note.
RNA from Tissues
Yield and Purity of RNA from Tissues
This data shows typical RNA yields from fibrous and nonfibrous mouse tissues using the ReliaPrep™ RNA Tissue Miniprep System. RNA was isolated from 10mg samples of various mouse tissues. Yield and purity were determined by absorbance spectroscopy using a NanoDrop® 1000 instrument.
More data illustrating use of the ReliaPrep™ RNA Tissue Miniprep System is available in this Application Note.
Protocols
Complete Protocol
Protocolos Rápidos
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130C | 2 × 10/pk | |
| ReliaPrep™ Minicolumns | A207A | 1 × 10/pack | |
| 1-Thioglycerol | A208A | 1 × 75μl | |
Column Wash Solution (CWE) |
A254A | 1 × 1ml | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
|
BL Buffer |
Z103A | 2 × 3.25ml | |
|
Elution Tubes |
Z200B | 1 × 25/pk | |
|
RNA Wash Solution (RWA) |
Z309B | 1 × 11.8ml | View Product |
| Yellow Core Buffer | Z317B | 1 × 300μl | |
|
MnCl2, 0.09M |
Z318A | 1 × 250μl | |
|
DNase I (lyophilized) |
Z358B | 1 × 1 vial | View Product |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130A | 2 × 50/pk | |
|
1-Thioglycerol |
A208B | 1 × 900μl | |
Column Wash Solution (CWE) |
A254B | 1 × 5ml | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
|
BL Buffer |
Z103B | 1 × 32.5ml | |
|
Elution Tubes |
Z200C | 1 × 50/pk | |
| RNA Wash Solution (RWA) | Z309E | 1 × 35ml | View Product |
|
Yellow Core Buffer |
Z317A | 1 × 2.5ml | |
|
MnCl2, 0.09M |
Z318A | 1 × 250μl | |
|
DNase I (lyophilized) |
Z358A | 1 × 1 vial | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130B | 2 × 250/pk | |
ReliaPrep™ Minicolumns |
A207B | 5 × 50/pack | |
|
1-Thioglycerol |
A208B | 2 × 900μl | |
Column Wash Solution (CWE) |
A254C | 1 × 24ml | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
| BL Buffer | Z103C | 1 × 150ml | |
|
Elution Tubes |
Z200C | 5 × 50/pk | |
|
RNA Wash Solution (RWA) |
Z309D | 1 × 206ml | View Product |
|
Yellow Core Buffer |
Z317C | 1 × 11ml | |
|
MnCl2, 0.09M |
Z318B | 2 × 750μl | |
|
DNase I (lyophilized) |
Z358A | 3 × 1 vial | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130C | 2 × 10/pk | |
| ReliaPrep™ Minicolumns | A207A | 1 × 10/pack | |
| 1-Thioglycerol | A208A | 2 × 75μl | |
Column Wash Solution (CWE) |
A254A | 1 × 1ml | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
Lysis Buffer (LBA) |
Z106A | 1 × 6ml | |
|
Elution Tubes |
Z200B | 1 × 25/pk | |
|
RNA Wash Solution (RWA) |
Z309B | 1 × 11.8ml | View Product |
| Yellow Core Buffer | Z317B | 1 × 300μl | |
|
MnCl2, 0.09M |
Z318A | 1 × 250μl | |
|
DNase I (lyophilized) |
Z358B | 1 × 1 vial | View Product |
|
RNA Dilution Buffer (RDB) |
Z372C | 1 × 9ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130A | 2 × 50/pk | |
|
1-Thioglycerol |
A208B | 2 × 900μl | |
Column Wash Solution (CWE) |
A254B | 1 × 5ml | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
Lysis Buffer (LBA) |
Z106B | 1 × 30ml | |
|
Elution Tubes |
Z200C | 1 × 50/pk | |
| RNA Wash Solution (RWA) | Z309E | 1 × 35ml | View Product |
|
Yellow Core Buffer |
Z317A | 1 × 2.5ml | |
|
MnCl2, 0.09M |
Z318A | 1 × 250μl | |
|
DNase I (lyophilized) |
Z358A | 1 × 1 vial | View Product |
|
RNA Dilution Buffer (RDB) |
Z372A | 1 × 44ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130B | 2 × 250/pk | |
ReliaPrep™ Minicolumns |
A207B | 5 × 50/pack | |
|
1-Thioglycerol |
A208B | 4 × 900μl | |
Column Wash Solution (CWE) |
A254C | 1 × 24ml | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
Lysis Buffer (LBA) |
Z106C | 1 × 150ml | |
|
Elution Tubes |
Z200C | 5 × 50/pk | |
|
RNA Wash Solution (RWA) |
Z309D | 1 × 206ml | View Product |
|
Yellow Core Buffer |
Z317C | 1 × 11ml | |
|
MnCl2, 0.09M |
Z318B | 2 × 750μl | |
|
DNase I (lyophilized) |
Z358A | 3 × 1 vial | View Product |
|
RNA Dilution Buffer (RDB) |
Z372B | 1 × 220ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
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