ReliaPrep™ RNA Miniprep Systems
Efficiently Purify RNA from as Few as 100 Cells or 0.25mg of Tissue
- One kit, two workflows: A single unified solution for both cell and tissue RNA extractions
- Simple and fast: Extract high-quality RNA in 30 minutes with a streamlined, easy-to-use protocol
- No phenol/chloroform or β-mercaptoethanol required
New: Cells and Tissue
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| Z6014 | ReliaPrep™ RNA Miniprep System | View Specifications | 50 preps | Consultar Precio | |||
| Z6015 | ReliaPrep™ RNA Miniprep System | View Specifications | 250 preps | Consultar Precio |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
Cells*
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| Z6010 | ReliaPrep™ RNA Cell Miniprep System | View Specifications | 10 preps | Consultar Precio | |||
| Z6011 | ReliaPrep™ RNA Cell Miniprep System | View Specifications | 50 preps | Consultar Precio | |||
| Z6012 | ReliaPrep™ RNA Cell Miniprep System | View Specifications | 250 preps | Consultar Precio |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
Tissue*
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| Z6110 | ReliaPrep™ RNA Tissue Miniprep System | View Specifications | 10 preps | Consultar Precio | |||
| Z6111 | ReliaPrep™ RNA Tissue Miniprep System | View Specifications | 50 preps | Consultar Precio | |||
| Z6112 | ReliaPrep™ RNA Tissue Miniprep System | View Specifications | 250 preps | Consultar Precio |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
*The ReliaPrep™ RNA Cell Miniprep Systems (Cat.# Z6010, Z6011, Z6012) are for cell samples only. The ReliaPrep™ RNA Tissue Miniprep Systems (Cat.# Z6110, Z6111, Z6112) are for tissue samples only. These are legacy configurations of the ReliaPrep™ RNA Miniprep System, which will be discontinued shortly and replaced by the consolidated ReliaPrep™ Miniprep Systems Cat.# Z6014, Z6015 listed above.
Recover High-Quality RNA from Cell and Tissue Samples Using a Single, Unified Kit
Precious samples and sensitive assays require RNA purification you can trust. The ReliaPrep™ RNA Miniprep System delivers consistent, high-purity RNA from both cultured cells and tissue samples using one streamlined kit designed for fast, reliable results.
The system enables preparation of intact total RNA in as little as 30 minutes. An optimized binding matrix efficiently captures RNA from very small or large input amounts, with elution volumes as low as 7–15µl—ideal for downstream assays that demand concentration and sensitivity.
Using this membrane-based purification workflow, you can process 100 to 5 × 10⁶ cultured cells or 0.25–20mg of tissue per prep. A built-in DNase treatment step removes contaminating DNA directly on the column, eliminating the need for phenol:chloroform extraction or ethanol precipitation. The result is clean, ready-to-use RNA suitable for a wide range of applications.
Note: The ReliaPrep™ RNA Miniprep System (Cat.# Z6014, Z6015) is the consolidated configuration of the legacy ReliaPrep™ RNA Cell Miniprep System (Cat.# Z6010, Z6011, Z6012) and ReliaPrep™ RNA Tissue Miniprep System (Cat.# Z6110, Z6111, Z6112). The legacy ReliaPrep™ RNA Cell Miniprep System and ReliaPrep™ RNA Tissue Miniprep System will be discontinued shortly.
Key Advantages
- One kit for all sample types: Cells and tissues are supported in a unified workflow.
- Low elution volume provides concentrated RNA that is ideal for precious or limited samples.
- High sensitivity for downstream assays, including those targeting low-copy transcripts.
- Fast protocol: Obtain purified RNA in ~30 minutes with minimal hands-on time.
- On-column DNase treatment removes genomic DNA without additional cleanup steps.
- Purified RNA is ready for common downstream applications, including RT-qPCR and sequencing.
RNA from Cells
Higher RNA Yields Across Diverse Cell Types and Culture Densities
The ReliaPrep™ RNA Miniprep System delivers consistent RNA yields across a wide range of cultured cell inputs, including low-input to high-density cultures and across a diverse range of cell types. RNA can be isolated from as few as 100 up to 1 × 10⁶ HeLa cells, with yields comparable to, or exceeding, those of the leading competitor. Robust RNA recovery can be obtained from 100,000 cells across four cell lines, demonstrating reliable performance even with low-input samples.
For additional data and performance details, see the full Application Note.
RNA from Tissue
Reliable Yield and Purity Across Challenging Tissue Types
The ReliaPrep™ RNA Miniprep System efficiently isolates RNA from a broad range of tissues, including both fibrous and nonfibrous sample types. The data shown below represent RNA yield from 10mg of mouse tissue samples, quantified using absorbance spectroscopy.
Purity metrics (A260/A280 and A260/A230 ratios) remain consistently high across tissue types, confirming clean RNA suitable for downstream applications such as RT-qPCR and sequencing.
Additional tissue performance data are available in the Application Note.
Protocols
Complete Protocol
Protocolos Rápidos
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
Column Wash Solution (CWE) |
A254B | 1 × 5ml | |
|
BL Buffer |
Z103B | 1 × 32.5ml | |
Lysis Buffer (LBA) |
Z106B | 1 × 30ml | |
| RNA Wash Solution (RWA) | Z309E | 1 × 35ml | View Product |
|
MnCl2, 0.09M |
Z318A | 1 × 250μl | |
|
RNA Dilution Buffer (RDB) |
Z372A | 1 × 44ml | |
|
Collection Tubes |
A130A | 2 × 50/pk | |
ReliaPrep™ Minicolumns |
A207B | 1 × 50/pack | |
|
1-Thioglycerol |
A208B | 2 × 900μl | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
|
Elution Tubes |
Z200C | 1 × 50/pk | |
|
Yellow Core Buffer |
Z317A | 1 × 2.5ml | |
|
DNase I (lyophilized) |
Z358A | 1 × 1 vial | View Product |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
Column Wash Solution (CWE) |
A254C | 1 × 24ml | |
| BL Buffer | Z103C | 1 × 150ml | |
Lysis Buffer (LBA) |
Z106C | 1 × 150ml | |
|
RNA Wash Solution (RWA) |
Z309D | 1 × 206ml | View Product |
|
Yellow Core Buffer |
Z317C | 1 × 11ml | |
|
RNA Dilution Buffer (RDB) |
Z372B | 1 × 220ml | |
|
Collection Tubes |
A130B | 2 × 250/pk | |
ReliaPrep™ Minicolumns |
A207B | 5 × 50/pack | |
|
1-Thioglycerol |
A208B | 4 × 900μl | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
|
Elution Tubes |
Z200C | 5 × 50/pk | |
|
MnCl2, 0.09M |
Z318B | 2 × 750μl | |
|
DNase I (lyophilized) |
Z358A | 5 × 1 vial | View Product |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130C | 2 × 10/pk | |
| ReliaPrep™ Minicolumns | A207A | 1 × 10/pack | |
| 1-Thioglycerol | A208A | 1 × 75μl | |
Column Wash Solution (CWE) |
A254A | 1 × 1ml | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
|
BL Buffer |
Z103A | 2 × 3.25ml | |
|
Elution Tubes |
Z200B | 1 × 25/pk | |
|
RNA Wash Solution (RWA) |
Z309B | 1 × 11.8ml | View Product |
| Yellow Core Buffer | Z317B | 1 × 300μl | |
|
MnCl2, 0.09M |
Z318A | 1 × 250μl | |
|
DNase I (lyophilized) |
Z358B | 1 × 1 vial | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130A | 2 × 50/pk | |
ReliaPrep™ Minicolumns |
A207B | 1 × 50/pack | |
|
1-Thioglycerol |
A208B | 1 × 900μl | |
Column Wash Solution (CWE) |
A254B | 1 × 5ml | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
|
BL Buffer |
Z103B | 1 × 32.5ml | |
|
Elution Tubes |
Z200C | 1 × 50/pk | |
| RNA Wash Solution (RWA) | Z309E | 1 × 35ml | View Product |
|
Yellow Core Buffer |
Z317A | 1 × 2.5ml | |
|
MnCl2, 0.09M |
Z318A | 1 × 250μl | |
|
DNase I (lyophilized) |
Z358A | 1 × 1 vial | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130B | 2 × 250/pk | |
ReliaPrep™ Minicolumns |
A207B | 5 × 50/pack | |
|
1-Thioglycerol |
A208B | 2 × 900μl | |
Column Wash Solution (CWE) |
A254C | 1 × 24ml | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
| BL Buffer | Z103C | 1 × 150ml | |
|
Elution Tubes |
Z200C | 5 × 50/pk | |
|
RNA Wash Solution (RWA) |
Z309D | 1 × 206ml | View Product |
|
Yellow Core Buffer |
Z317C | 1 × 11ml | |
|
MnCl2, 0.09M |
Z318B | 2 × 750μl | |
|
DNase I (lyophilized) |
Z358A | 3 × 1 vial | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130C | 2 × 10/pk | |
| ReliaPrep™ Minicolumns | A207A | 1 × 10/pack | |
| 1-Thioglycerol | A208A | 2 × 75μl | |
Column Wash Solution (CWE) |
A254A | 1 × 1ml | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
Lysis Buffer (LBA) |
Z106A | 1 × 6ml | |
|
Elution Tubes |
Z200B | 1 × 25/pk | |
|
RNA Wash Solution (RWA) |
Z309B | 1 × 11.8ml | View Product |
| Yellow Core Buffer | Z317B | 1 × 300μl | |
|
MnCl2, 0.09M |
Z318A | 1 × 250μl | |
|
DNase I (lyophilized) |
Z358B | 1 × 1 vial | View Product |
|
RNA Dilution Buffer (RDB) |
Z372C | 1 × 9ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130A | 2 × 50/pk | |
ReliaPrep™ Minicolumns |
A207B | 1 × 50/pack | |
|
1-Thioglycerol |
A208B | 2 × 900μl | |
Column Wash Solution (CWE) |
A254B | 1 × 5ml | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
Lysis Buffer (LBA) |
Z106B | 1 × 30ml | |
|
Elution Tubes |
Z200C | 1 × 50/pk | |
| RNA Wash Solution (RWA) | Z309E | 1 × 35ml | View Product |
|
Yellow Core Buffer |
Z317A | 1 × 2.5ml | |
|
MnCl2, 0.09M |
Z318A | 1 × 250μl | |
|
DNase I (lyophilized) |
Z358A | 1 × 1 vial | View Product |
|
RNA Dilution Buffer (RDB) |
Z372A | 1 × 44ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130B | 2 × 250/pk | |
ReliaPrep™ Minicolumns |
A207B | 5 × 50/pack | |
|
1-Thioglycerol |
A208B | 4 × 900μl | |
Column Wash Solution (CWE) |
A254C | 1 × 24ml | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
Lysis Buffer (LBA) |
Z106C | 1 × 150ml | |
|
Elution Tubes |
Z200C | 5 × 50/pk | |
|
RNA Wash Solution (RWA) |
Z309D | 1 × 206ml | View Product |
|
Yellow Core Buffer |
Z317C | 1 × 11ml | |
|
MnCl2, 0.09M |
Z318B | 2 × 750μl | |
|
DNase I (lyophilized) |
Z358A | 3 × 1 vial | View Product |
|
RNA Dilution Buffer (RDB) |
Z372B | 1 × 220ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
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